Souvik Maiti Lab is situated at CSIR-Institute of Genomics and Integrative Biology. We are interested in unusual nucleic acid structures, their biological roles in non-coding RNA and targeting these by chemically engineered oligonucleotides or small molecules. We use biophysics, molecular biology and zebrafish biology as a tool in our study. We also focus on CRISPR-Cas based gene editing and diagnostics (CRISPRDx FELUDA) for development of diagnostic tests for genetic diseases like Sickle Cell Anemia (SCA) and infectious diseases live COVID-19 along with exploring potential gene editing approaches to correct disease-causing mutations.
Using a combination of biophysical, biochemical and molecular biology methods coupled to computational predictions, we seek to:
Study RNA G quadruplexes and their influence on regulation of gene expression.
Identify small molecule modulators of miRNA function as potential therapeutic leads.
Study the relationship between lncRNA structure and function.
Develop CRSIPR-Cas based diagnostics
Some of the research methods that we deploy in the lab include:
CRISPR-Cas based gene editing and diagnostics
Gel shift assays (DNA + RNA)
Circular dichroism spectropolarimetry
Surface plasmon resonance (Biacore 2000)
Isothermal titration calorimetry
Differential scanning calorimetry
Confocal Microscope-with FCS (LSM 510-Zeiss)