Souvik Maiti Lab is situated at CSIR-Institute of Genomics and Integrative Biology. We are interested in unusual nucleic acid structures, their biological roles in non-coding RNA and targeting these by chemically engineered oligonucleotides or small molecules. We use biophysics, molecular biology and zebrafish biology as a tool in our study. We also focus on CRISPR-Cas based gene editing and diagnostics (CRISPRDx FELUDA) for development of diagnostic tests for genetic diseases like Sickle Cell Anemia (SCA) and infectious diseases live COVID-19 along with exploring potential gene editing approaches to correct disease-causing mutations.

Using a combination of biophysical, biochemical and molecular biology methods coupled to computational predictions, we seek to:

  • Study RNA G quadruplexes and their influence on regulation of gene expression.

  • Identify small molecule modulators of miRNA function as potential therapeutic leads.

  • Study the relationship between lncRNA structure and function.

  • Develop CRSIPR-Cas based diagnostics

Some of the research methods that we deploy in the lab include:

    • CRISPR-Cas based gene editing and diagnostics

    • Molecular biology

    • Gel shift assays (DNA + RNA)

    • UV-VIS Spectroscopy

    • Fluorescence Spectroscopy

    • Circular dichroism spectropolarimetry

    • Surface plasmon resonance (Biacore 2000)

    • Isothermal titration calorimetry

    • Differential scanning calorimetry

  • Confocal Microscope-with FCS (LSM 510-Zeiss)